Chitosan osteoarthritis gene delivery

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August undefined, 2024

WebSep 24, 2024 · 2.1. Solubility. Chitosan is produced by deacetylation of chitin; in this process, some N-acetylglucosamine moieties are converted into glucosamine units.The presence of large amounts of protonated -NH 2 groups on the chitosan structure accounts for its solubility in acid aqueous media since its pKa value is approximately 6.5 [].When … WebMar 3, 2006 · All these studies point to the versatility and promise of chitosan as a gene carrier, although only limited in vivo efficacy has been demonstrated. Due to the complex …

(PDF) Intra-articular Injection of Chitosan-Based Supramolecular ...

WebNov 15, 2024 · (1) Background: Collagen is the main component of the connective tissue, playing an important role in the histological architecture and function of living organisms. Targeted therapy and improved imaging diagnosis can be obtained through collagen-binding nanoparticles that concentrate in the extracellular matrix. (2) Methods: We performed a … WebJan 2, 2014 · The development of safe and efficient gene carriers is the key to the clinical success of gene therapy. The present study was designed to develop and evaluate the chitosan-graft-polyethylenimine (CP)/DNA nanoparticles as novel non-viral gene vectors for gene therapy of osteoarthritis. florists \u0026 gift shops in spring hill

Chitosan for gene delivery: Methods for improvement …

WebJan 1, 2024 · Thermoresponsive DDS, chitosan-modified MoS 2 (molybdenum disulfide), was shown to release a small molecule drug, dexamethasone (Dex) ... is most commonly employed osteoarthritis therapeutic for gene delivery; currently there are two ongoing trials for intra-articular delivery using an AAV (NCT02790723) and an HDAV … WebChitosan can act itself as a precursor or carrier for medical applications such as drug delivery, gene delivery, and wound healing. With the advancement in science … WebDec 1, 2016 · Efficacy of the combination of CM-cordycepin and HAMA hydrogel as a treatment for OA. Safranin-O staining of cartilage samples 4 weeks (A) and 8 weeks (B) after OA induction. greece physical environment

Novel hyaluronic acid–chitosan nanoparticles as non-viral …

WebFeb 23, 2024 · Keywords: Osteoarthritis, Gene therapy, Chitosan, Hyaluronic acid, Chondroitin sulfate, Growth and differentiation factor-5 (GDF-5) plasmid. 1. ... HA and CHS enabled the non-viral vector itself to have some therapeutic effects on OA, making it much more suitable for delivery of gene therapy treatments for OA. WebConclusions: These results suggested that HA/CS-CrmA nanoparticles could attenuate cartilage destruction and protect against early OA by inhibiting synovial inflammation via … greece pilot bookWebJan 1, 2024 · The chitosan-based gene delivery system has several characteristics such as insignificant cellular toxicity, high gene delivery ability, targeting of particular cells, … florists \u0026 gift shops in tallahassee

"WebAug 31, 2024 · Although gene delivery systems can be viral or non-viral, non-viral gene delivery materials are being specifically developed that could deliver the gene as a safer … " - Chitosan osteoarthritis gene delivery

Chitosan osteoarthritis gene delivery

WebNov 28, 2011 · Gene therapy, which treats the causes of osteoarthritis by targeting specific pathological mechanisms, is attracting much attention as a promising new treatment … WebJan 2, 2014 · Abstract. The development of safe and efficient gene carriers is the key to the clinical success of gene therapy. The present study was designed to develop and …

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WebJun 1, 2024 · Modifying chitosan with target ligands is a promising method for the targeted delivery of genes by delivery systems based on chitosan. Proteins, peptides, aptamers, carbohydrates, and small molecules are common ligands used to realize target delivery in gene vectors based on chitosan. WebMar 3, 2006 · Next, chitosan–DNA nanoparticles containing IL-1Ra or IL-10 gene were injected directly into the knee joint cavities of osteoarthritis rabbits to clarify the in vivo …

WebJan 28, 2024 · Methods In this study, an injectable and self-healing hydrogel was synthesized by in situ crosslinking of N -carboxyethyl chitosan ( N -chitosan), adipic acid dihydrazide (ADH), and hyaluronic... WebApr 10, 2024 · Novel hyaluronic acid–chitosan nanoparticles as non-viral gene delivery vectors targeting osteoarthritis. International Journal of Pharmaceutics, 420 (2) (2011) ... Combining hyaluronic acid with chitosan enhances gene delivery. Journal of Nanomaterials (2014), 10.1155/2014/246347. 2014. Article 152. Google Scholar.

WebDec 17, 2024 · To construct a novel non-viral vector loaded with growth and differentiation factor-5 (GDF-5) plasmid using chitosan, hyaluronic acid, and chondroitin sulfate for osteoarthritis (OA) gene therapy. Methods Nano-microspheres (NMPs) were prepared by mixing chitosan, hyaluronic acid, and chondroitin sulfate. WebOsteoarthritis (OA) is a painful, degenerative and inflammatory disease that affects the entire synovial joints. Nowadays, no cure exists, and the pharmacological treatments …

WebApr 11, 2024 · 1.3.1.Advantageous properties of chitosan as a biomaterial. Chitosan has a structure similar to that of glycine aminoglycan, which is widely distributed in connective tissue, giving chitosan good biocompatibility (Malafaya et al., 2007).Chitosan-based biomaterials do not cause cytotoxicity or genotoxicity and promote cell proliferation and …

WebThingreila Muinao, ...Mintu Pal, in Polysaccharide Carriers for Drug Delivery, 2024. 15.4.2.9 Chondroitin sulfate. Chondroitin sulfate is a natural water soluble polymer comprising of repeating disaccharide units of d-glucuronic acid and N-acetylgalactosamine, sulfated at either 4- or 6-positions.While cross-linked chondroitin sulfates may show less hydrophilic … greece phrasesWebJan 28, 2024 · A The optical images of the gelation progress. The mixture of N-chitosan and ADH presented in the sol state at room temperature, and it experienced sol-gel … greece physical geography mapWebMar 6, 2024 · Chitosan as a gene delivery vector should provide stability to vector-cargo complexes, protecting them from extracellular and intracellular degradation, delivering … florists \u0026 gift shops in sanduskyWebdesigned to develop and evaluate the chitosan-graft-polyethylenimine (CP)/DNA nanoparticles as novel non-viral gene vectors for gene therapy of osteoarthritis. florists \u0026 gift shops in saffordWebChitosan can act itself as a precursor or carrier for medical applications such as drug delivery, gene delivery, and wound healing. With the advancement in science technology, it was clearly observed that the amine groups present in chitosan are responsible for various properties such as antimicrobial activity, stability in a wide pH range ... greece physical characteristicsWebJun 23, 2024 · Nanoparticle-based local gene transfer can alter the expression of the endogenous genes to prevent or slow the pathological progress of OA by introducing genes, such as DNA, mRNA, siRNA, and miRNA, into the target cells. florists \u0026 gift shops in thomasvilleWebThere are two main approaches for gene delivery using viral and non-viral gene transfer systems for treating articular cartilage defects in OA, or following joint trauma. The first is … florists \u0026 gift shops in seattle