Phospholipidosis detection reagent
WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is … WebJul 16, 2015 · For phospholipid staining, the cells were treated with 400 μM palmitate containing 1X LipidTOX Red phospholipidosis detection reagent. For Immunostaining with an anti-calnexin antibody, the cells were fixed in 4 % paraformaldehyde in PBS. The fixed cells were rinsed with PBS and incubated in blocking solution (5 % goat serum, and 0.001 …
Phospholipidosis detection reagent
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WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. WebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis. Cell Analysis, Cellular Imaging, High-Content Screening (HCS) Order Info Shipping Condition: Room temperature Specifications
WebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected … WebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological …
WebJan 27, 2024 · Briefly, cells were incubated in complete bronchial epithelial growth medium supplemented with 1 x phospholipidosis detection reagent for 48 hr. Cells were subsequently fixed in 4% PFA and stained with 1 X neutral lipid detected reagent for 30 min and counterstained with 1 μg mL-1 Hoechst 33258. Staining intensity was normalized to … WebJan 1, 2014 · Phospholipidosis (PLD) is characterized by excessive intracellular accumulation of phospholipids, with a distinct morphological appearance of lamellar bodies visible under an electron microscope (EM). 1 Drugs, including many antibiotics, antidepressants, and antihistamines, induce PLD in various tissues such as lung, 2 heart, …
WebJun 6, 2024 · The phospholipid storage was accompanied by an expansion of the endolysosomal compartment in cultured TEC as evidenced by the increased intensity of LysoTracker Red staining and the enhanced...
WebDec 17, 2016 · Drug-induced phospholipidosis caused by combinations of common drugs in vitro.药物引起phospholipidosis引起的常见药物在体外 ... combination limitmea- surement mistakes. cellswere further24 concomitantviability detection, 11 PrestoBlueCellViability Reagent (PrestoBlue) (Invitrogen, Carlsbad, US) were added eachwellafter 22 hto ... bing vs google statisticsWebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader equipped with filters that can detect excitation/emission maxima ∼595/615 nm. bing vs google search usageWebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected drugs. According to the manufacturer's protocol, LipidTOX was diluted in full media (1:500) and then filtered with a syringe filter (2 μm in pores size). ... dab oil thcWebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. This reagent is easily detected by fluorescence microscopy or an HCS reader … The HCS LipidTOX™ Red phospholipidosis detection reagent was developed to … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes daboii on the radarWebSep 3, 2012 · Drug-induced phospholipidosis (PLD) is a lysosomal storage disorder characterized by the accumulation of phospholipids within the lysosome. ... After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their … bing vs google usage statistics 218WebFeb 27, 2024 · The LipidTOX Green Phospholipidosis Detection Reagent is dedicated to phospholipidosis detection, which is often triggered by cationic amphiphilic drugs. Within 24 h of incubation with CQ, the increase in fluorescence intensity of LipidTOX for phospholipidosis detection was significant. bing vs google search qualityWebdrug-induced phospholipidosis. The morphological hallmark of phospholipidosis is the appearance of unicentric or multicentric-lamellar bodies when viewed under an electron … bingwa initiative