WebDec 14, 2024 · Patient and reference DNA were processed as specified by manufacturer protocols. ... (Invitrogen) was incubated with BSA (220 µg), sonicated herring sperm DNA (40 µg) ... WebRockland Immunochemicals Inc. This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded….
Fragmentation of DNA by sonication - PubMed
WebThe chromosome-specific repetitive DNA probes were denatured for 10 min at 72C in a hybridization mixture containing 2 μg/ml probe DNA, 500 μg/ml sonicated herring sperm DNA (Sigma), 0.1% Tween-20, 10% dextran sulfate, and 60% formamide in 2 × SSC at pH 7.0. Webhybridized in 1 M NaCl, 0.1% SDS, 1.5 mg/mL herring sperm DNA, and 10% dextran for 3 hours at 65°C and then hybridized with the appropriate radiolabeled probe at 65°C for 12-24 hours in the prehybridization solution with the addition of 1.5 mg/mL sonicated herring sperm DNA. Blots were washed to various stringencies, depend- saying for church signs for fall
DNA Library - an overview ScienceDirect Topics
WebSDS, 75 mmol/L EDTA and 0.1 mg denatured sonicated herring sperm DNA per milliliter. Hybridization with the gamma 32P 5' end-labeled oligonu- cleotide probe was performed at 42" C for 16 hr. Blots were washed twice for 15 min at 25" C and once for 15 min at 50" C in 2 x SSC/O.Ol% SDS. Autoradiography was performed for 4 Webtained sonicated herring sperm DNA at 250-500 ,/g/ml. Hy-bridization buffer was 35-50% (vol/vol) in deionized form-amide and contained 250-500 ,g of sonicated DNA per ml. Filters were subjected to prehybridization treatment for 2-4 hr at 42?C and were hybridized at 42?C under probe concentration and time conditions given in Results. WebDec 1, 2001 · dNTPs and excess sonicated herring sperm DNA (0.5 mg/ml) as a trap (lanes 3 and 4). After incubation for10 min at 37 ° C , samples were quenched and run on a 10% polyacrylamide gel. scalp\\u0027s sh